Announcement about the defense of the Doctor of Philosophy (Ph.D.) dissertation of Khidoyatova Shakhnoza Komil qizi

I. General information.

The topic of the dissertation, specialization code (name of the industry in which the degree is awarded): “Lipids of plants of the genera Matricaria, Calendula, Ajuga, Lycopersicon and the development of drugs based on them” 02.00.10 – Bioorganic chemistry (chemical sciences).

Registration number of the dissertation topic: В2023.1.PhD/К600

Scientific supervisor: Gusakova Svetlana Dmitrievna, Doctor of sciences in chemistry, professor.

Name of the institution where the dissertation was fulfilled: Institute Chemistry of Plant Substances.

The institution where Scientific Council operates, Scientific Council number: Institute Chemistry of Plant Substances, DSc. 02/30.01.2020.K/T.104.01.

Official opponents: Boboev Bakhrom Nurillaevich, Doctor of sciences in chemistry, Normaxamatov Nodirali Soxobotalievich, Doctor of sciences in chemistry.

Leading organization: Institute of Bioorganic Chemistry

Direction of the dissertation: practical.

II. The purpose of the research is to study the lipids of the plants Matricaria recutita, Matricaria matricarioides, Calendula officinalis, Ajuga turkestanica and Lycopersicon esculentum, to develop methods for obtaining oil extracts, and to create new drugs based on oil extracts, plant phospholipids and carotenoids.

III. Scientific novelty of the research: lipids of the seeds of the plant Matricaria matricarioides were studied for the first time, epoxy- and hydroxyacyldiacylglycerides were found in their composition; Using physical spectroscopy, chemical and chromatographic methods, the composition and structure of oxygenated fatty acids were determined and it was established that they are dominated by known isomeric acids 9 ,10-epoxy-18:1(12), 12,13-epoxy-18:1(9), 9-hydroxy-18:2(10.12) and 13-hydroxy-18:2(9.11) , specific for lipids of the family Asteraceae;

for the first time lipids of the leaves, flowers, roots and seeds of the plant Ajuga turkestanica were studied; the set of components and composition of fatty acids of neutral lipids, glyco- and phospholipids have been established; in the phospholipids of all organs, iridoids and ecdysterone, co-extracted with these lipids and characteristic of A. turkestanica, were found;

the lipids of the tomato peel (Lycopersicon esculentum) were characterized and for the first time it was shown that the peel is enriched with carotenoids with a high content of lycopene and contains a complex of biologically active lipid components (ethyl esters of fatty acids, phytosterols, triterpenols);

has been developed a method for ultrasonic two-phase alcohol-oil extraction of lipids from the vegetative organs of medicinal plants, which makes it possible to obtain oil, aqueous-alcohol and alcohol extracts with a high content of biologically active substances;

a new phyto-oil “Biofitol” was created from oil extracts of Matricaria recutita inflorescences, Calendula officinalis and Ajuga turkestanica, which stimulates the regenerative processes of the body and promotes the effective treatment of diseases of the gastrointestinal tract;

It has been established that the developed composition of the hepatoprector drug “Hepalipin”, based on soy lecithin, unrefined sunflower oil, carotenoid extract Lycopersicon esculentum, ecdysthene and glycyrrhizic acid, exhibits a more pronounced hepatoprotective effect compared to the foreign drug “Phosphogliv” (Russian Federation).

IV. Implementation of research results. Based on the results obtained on the research of lipids and lipophilic compounds of the plants Matricaria matricarioides, Matricaria recutita, Calendula officinalis, Ajuga turkestanica and Lycopersicon esculentum growing in Uzbekistan:

a patent from the Intellectual Property Agency of the Republic of Uzbekistan (No. IAP 04702, 2013) was received for a method of complex processing of plant raw materials. The results of scientific research made it possible to obtain oil, hydroalcoholic and alcoholic extracts with a high content of biologically active substances;

a patent was received from the Intellectual Property Agency of the Republic of Uzbekistan for a method for obtaining a product that stimulates the body’s regenerative processes (№ IAP 05367, 2017). The results made it possible to obtain new herbal oils based on medicinal plant extracts, enriched with biologically active substances with a wide pharmacological spectrum;

a temporary pharmacopoeial monograph was compiled and approved for the substance of the new hepatoprotective agent “Hepalipin” (TPA 42 Uz-4691-2021 dated 11/10/2021). As a result of the research, it was possible to introduce a substance with hepatoprotective action into practice.

Hepalipin drug substance has been registered in the Ministry of Health of the Republic of Uzbekistan (No. DV/M 03937/10/21 dated 11/10/2021). This allows the use of this drug in medical practice and the production of the drug substance;

permission was received from the Ministry of Health of the Republic of Uzbekistan to conduct clinical trials of the drug “Hepalipin” and a clinic in Tashkent was identified to conduct clinical trials of the drug (certificate of the Pharmacological Committee No. 29/03-529 dated July 22, 2022). As a result, this makes it possible to import substitution of this type of medicine in the republic.

Print