Announcement of the thesis defense of the Doctor of Philosophy (PhD) Makhmudova Shokhista Rakhimjonovna

Announcement of the thesis defense of the Doctor of Philosophy (PhD) Makhmudova Shokhista Rakhimjonovna

I. General information.

The topic of the dissertation, the cipher of the specialty (the name of the branch of science in which the academic degree is awarded): “Chemical Composition and Properties of Crocus sativus and Senecio subdentatus plants of the flora of Uzbekistan”, 02.00.10 – Bioorganic chemistry.

The registration number of the thesis topic: В2024.4.PhD/K892.

Scientific supervisor: Leading Researcher, Doctor of Chemical Sciences Nishanbayev Sabir Zaripbaevich.

The name of the institution where the dissertation was performed: S.Y. Yunusov Institute of the Chemistry of Plant Substances, Academy of Sciences of the Republic of Uzbekistan.

The name of the institution where the Scientific council works, the number of the scientific council: S.Y. Yunusov Institute of the Chemistry of Plant Substances, Academy of Sciences of the Republic of Uzbekistan, DSc.05/2025.27.12.K/T.20.01.M.

Official opponents: Doctor of Chemical Sciences, professor Gafurov Makhmud Bakiyevich, Doctor of Chemical Sciences Jo‘raqulov Sherzod Niyatqobulovich.

Leading organization: National University of Uzbekistan.

II. The aim of the study are.

To conduct phytochemical investigations of Crocus sativus and Senecio subdentatus growing in Uzbekistan and to evaluate their biological activities.

III. Scientific novelty of the research:

The essential oils of Crocus sativus and Senecio subdentatus from Uzbekistan were comprehensively analyzed for the first time, resulting in the identification of 67 and 72 volatile mono-, sesqui-, and diterpenoids, respectively.

From saffron petals, four phenolic acids and six flavonoids were isolated; from stamens, sixteen flavonoids and two nitrogen-containing heterocyclic compounds were isolated. Compounds such as tiliroside, isorhamnetin-3-O-neohesperidoside, rhamnetin-3-O-β-D-Glcp, brassicin, and flazine were isolated from the genus Crocus for the first time.

From Senecio subdentatus, four phenolic acids and seven flavonoids were identified for the first time.

The crystal structures and absolute configurations of kaempferol-3-O-sophoroside and helichrysoside were determined by X-ray diffraction.

The lipid composition of saffron petals and stamens was investigated for the first time, revealing high carotenoid content, confirming stamens as a rich carotenoid source.

Pharmacological studies demonstrated strong antioxidant, antihypoxic, hypolipidemic, anti-inflammatory, and hepatoprotective activities of flavonoid fractions.

IV. Implementation of the research results. Based on the investigation of the chemical composition and properties of Crocus sativus and Senecio subdentatus plants:

a technological guideline has been developed for the production of the biologically active food supplement “Saflavin”, possessing antioxidant, antihypoxic, anti-inflammatory, and hypolipidemic activities, formulated on the basis of the total flavonoid fraction isolated from the petals of Crocus sativus. The technological documentation was approved by the Committee for Sanitary and Epidemiological Welfare and Public Health of the Republic of Uzbekistan (Technical Specification No. 03535440-070:2025, dated 24.06.2025). In addition, a production authorization certificate was obtained (No. 1313274; Registration No. R-153412402, 13.01.2026). As a result, the opportunity has been established for the industrial production of a biologically active food supplement exhibiting antioxidant, antihypoxic, anti-inflammatory, and hypolipidemic effects;

The single-crystal X-ray diffraction data of the isolated compounds kaempferol-3-O-sophoroside and helichrysozide were deposited in the Cambridge Structural Database CSD, Cambridge, United Kingdom; ccdc.cam.ac.uk/structures/) and officially registered. The corresponding CCDC deposition numbers (CCDC  2486410; 2465519), as well as ENOKUR host ID numbers, were assigned. As a result, the deposition of these data in the electronic database enables the determination of the three-dimensional molecular structures of related compounds and facilitates the prediction of their potential pharmacological activities using specialized computational software.

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