PHYTOECDYSTEROIDS OF PLANTS OF THE GENERA SILENE (TOMENTELLA, POPOVII, CLAVIFORMIS), DIANTHUS HELENAE AND THEIR BIOLOGICAL ACTIVITY

INTRODUCTION (abstract of the PhD thesis)

The aim of the research work is isolation the phytoecdysteroids of Silene tomentella (Schischk.), Silene popovii (Schischk.), Silene claviformis (Litv.), and Dianthus helenae (Vved.) plants, determine their chemical structure and study of their biological activities.

The objects of the research work are phytoecdysteroids of the S. tomentella, S. popovii, S. claviformis, and D. helenaе plant species growing in the flora of Uzbekistan.

The scientific novelty of the research is as follow:

for the first time, 9 known phytoecdysteroids and one new compound - 20- hydroxyecdysone 25-O-benzoate were isolated from the Silene popovii plant and its structure was proved;

for the first time, 2 known phytoecdysteroids have been isolated from Silene tomentella and their structure identified;

for the first time, 6 known phytoecdysteroids were isolated from Dianthus helenae plant and 2 from Silene claviformis plant, and their chemical structure was studied;

The diversity of representatives of Silene, Ajuga, Rhaponticum species was analyzed, distribution of more than 120 phytoecdysteroids of more than 184 Silene, Ajuga, Rhaponticum species in plants, chemical structure, and isolated sources were systematized, based on the results monograph was prepared, and published named after "Phytoecdysteroids: Properties, Biological Activity and Applications".

Implementation of the research results. Based on the scientific results of the study of the chemical composition and biological activities of S. tomentella, S. claviformis, S. popovii and Dianthus helenae plants:

normative and technical documents standard organization (Ts 03535440- 036:2020, 01.06.2020) and technological institution (TИ 03535440-32: 2018,

10.09.2020) for biologically active supplement (BAS) "Diantrop" with nootropic activity have been developed and approved by the Sanitary-epidemiological peace and public health service of the Republic of Uzbekistan as a result, it was possible to develop BAS with nootropic effect based on local raw materials;

for a phytoecdysteroid with hypoglycemic activity ((2R,3R)-2-((1S,2S,3R,5R,10R,13R,14S,17S)-2,3-diacetoxy-1,14-dihydroxy-10,13- dimethyl- 6-oxo-2,3,4,5,6,9,10,11,12,13,14,15,16,17-tetradecahydro-1Hcyclopenta[a]phenanthren-17-yl)-6 -hydroxy-6-methylheptane-2,3-diyl diacetate, obladyushchee hypoglycemic activity) received a patent for the invention of the Intellectual Property Center of the Republic of Uzbekistan (№IAP 06824, 28.03.2022);

from the results of researching the chemical composition and biological activities of plants of the Silene and Dianthus genera, foreign scientists published scientific works in journals with more than 10 high impact factors in the chemical and quantitative description of primary and secondary metabolites isolated from plants, as well as against microbes, fungi, diabetes and stress. used to describe their activity (RSC Advances, 2022, 12, 5031, https://doi.org/10.1039/D1RA07273K,

IF- 4.03; Frontiers in Pharmacology, 2022, 18,42

https://doi.org/10.3389/fphar.2022.998965, IF- 5.98; International Journal of Molecular Sciences, 2022, 23, 8364, https://doi.org/10.3390/ijms23158664, IF- 6.20, etc.). The results made it possible to obtain information about the chemical composition and biological activities of plants of the Silene and Dianthus genera;

information on the chemistry, biological activity and application of phytoecdysteroids in the monograph, which is included 7 chapters "Phytoecdysteroids: Properties, Biological activity and Applications" published by